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New PDF release: Arachidonate Related Lipid Mediators

By John N. Abelson, Melvin I. Simon, Robert C. Murphy, Frank A. Fitzpatrick

ISBN-10: 0121820882

ISBN-13: 9780121820886

This quantity comprises info on arachidonic acid metabolites and platelet activating issue. Assays for the quantitative dimension of prostaglandins, leukotrienes, and platelet-activating issue, together with enzyme immunoassays and mass spectrophotometric thoughts, are offered. guidance and research of cytochrome P-450 metabolites of arachidonic acid are mentioned. The isolation, purification, and assay of enzymes vital within the biosynthesis and metabolism of those lipids are incorporated

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Healy, J. A. Lawson, and G. A. FitzGerald, Proc. Natl. Acad. Sci. A. 83, 5861 (1986). 42 ASSAYS [5] urine, together with a fractional elimination comparable to that of 2,3dinor-TxB2, make these measurements an extremely convenient approach to monitoring thromboxane production both in healthy and disease conditions. The unique chemical properties of 11-dehydro-TxB2 are associated with greater than usual specificity of the immunological recognition, which combined with adequate chromatographic procedures, can allow reliable assessment of metabolite levels in complex biological fluids.

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Barrow, K. A. Waddell, M. Ennis, C. T. Dollery. and I. A. Blair, J. Chromatogr. ) 3 I. A. Blair, Br. Med. Bull. 39, 223 (1983). 4 K. A. Waddell, I. A. Blair, and J. WeUby, Biomed. Mass Spectrum. 10, 83 (1983). 5 R. J. Strife and R. C. Murphy, J. Chromatogr. 305, 3 (1984). 6 C. S. Ramesha and W. C. Pickett, Biomed. Environ. Mass Spectrom. 13, 107 (1986). 7 C. R. Pace-Asciak, Adv. Prostaglandin Thromboxane Leukotriene Res. 18, 1 (1989), METHODS IN ENZYMOLOGY, VOL. 187 Copyright © 1990 by Academic Press, Inc.

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Arachidonate Related Lipid Mediators by John N. Abelson, Melvin I. Simon, Robert C. Murphy, Frank A. Fitzpatrick

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